FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15367545
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT291780.18986767242262834No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC156210.10164928750818689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA69800.043.14619
GTGCGCG88650.034.6683588
TTGAACG33000.030.37237
CGACCTA16050.030.186234
TAACACG115500.027.9234242
AACACGT122300.026.4486163
ACGTGCG126400.025.6674526
ACACGTG149350.021.5947534
CGCGAGT73800.021.5621810-11
GAACGTA17500.021.442374
CTAACAC160950.021.011781
CGAGTCG77500.020.53202412-13
CGTGCGC164600.020.1146347
GTATCGA24650.019.8474184
TCGAACG23600.019.825540-41
AACGTAT18950.019.8016595
CACGTGC171450.018.894295
GAACGCT54050.018.8072729
ATTGAAC61950.018.6326276
CCCGTAT28550.018.1340241
Produced by FastQC (version 0.11.5)