FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8094070
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT159460.19700842715716566No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC93110.11503483414400913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA37500.044.9626929
GTGCGCG46550.036.7314538
TTGAACG15450.029.204547
GAACGTA6000.025.3310974
ACGTGCG88200.022.67096
AACACGT90950.022.0376363
AACGTAT7000.021.7123685
CGCGAGT42850.021.61419510-11
TAACACG95300.020.9818822
CGAGTCG44850.020.6503512-13
ACACGTG103550.018.989154
ACGTATT7800.018.8765376
CTAACAC112800.018.6025091
CACGTGC107550.018.5478745
CGACCGT5400.018.5040821
CGTGCGC110350.018.4646137
ATTGAAC30350.017.5272836
CTCAGAT56650.017.2185251
CGTATTC8600.017.1205837
TCGCACG53800.016.99431624-25
Produced by FastQC (version 0.11.5)