FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8311191
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT162850.19594062992897165No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC95600.11502563230709052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA39450.042.4991579
GTGCGCG47400.036.0725448
GAACGTA5350.027.5207654
CGACCGT5250.027.190021
TTGAACG15850.025.4707817
AACGTAT6650.023.56925
ACCGTTG5850.022.7328953
ACGTGCG93500.022.2492356
CGCGAGT41950.021.96455410-11
AACACGT94500.021.5111963
CGAGTCG44600.020.76597612-13
TAACACG98650.020.6062662
ACACGTG103900.019.656474
GTATCGA11200.019.5070884
CACGTGC108850.019.3734575
ACGTATT7750.018.9982056
CGTGCGC113750.018.5806647
CTAACAC115950.018.3025531
TCGCACG52450.017.79383924-25
CTCAGAT58450.017.0955221
Produced by FastQC (version 0.11.5)