FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8311191
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT120050.14444379872872612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA37200.039.0647289
GTGCGCG45900.032.073978
TTGAACG17250.025.3296157
GAACGTA6200.022.2221074
AACACGT84750.022.1991843
ACGTGCG84850.021.829446
TAACACG89250.021.2607252
CGCGAGT39900.019.28183710-11
ACACGTG96200.019.260544
CTAACAC108950.018.9012181
CACGTGC101450.018.4019785
CGAGTCG42400.018.14449912-13
CGTGCGC106000.017.6082637
ATTGAAC31950.017.540466
CTCAGAT59200.017.0311241
CGACCGT6500.016.828431
AACGTAT7900.016.2353135
GAACGCT27950.015.97174559
GTATCGA11900.015.5702864
TCGAACG13750.015.54055340-41
Produced by FastQC (version 0.11.5)