FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004922967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25025193
Sequences flagged as poor quality0
Sequence length150
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT791730.3163731844146017No Hit
CTTCTGGGATGTCCGCTGCAATCTGCTCCGGGGCTGCAGCAACCTCATCA449750.17971889367646438No Hit
TCCCAGAAGTGGTTGTTTCCCTTGCATGGGACGAAAGCTTGGCTCCAAAG406900.1625961486091236No Hit
ACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCT394760.1577450371711419No Hit
CTCCTGCAATGCACGCTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTT357300.14277612164669418No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336410.13442853367804197No Hit
TCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAA306830.12260844501778667No Hit
TCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTG262450.10487431605422583No Hit
GCCTAGCTAGAGGATCTGTGACCCCAGCCATGAGGACCCTCGCCATCCTT258160.10316004356090282No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)