FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5833075
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385590.6610407032311431No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170380.2920929355442884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139900.23983919287854177No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA308050.072.725021
GGTATCA127200.070.075771
TATCAAC411700.054.2811362
ATCAACG424350.052.8870433
TCAACGC430600.052.2987944
CAACGCA432200.052.022255
AACGCAG442400.050.996556
ACGCAGA462700.048.6817177
CGCAGAG478850.047.051458
GCAGAGT502050.044.9008459
AGAGTAC487600.033.7475210-11
CAGAGTA496800.031.65151410-11
GAGTACT222700.028.30735812-13
AGTACGG365950.027.34163712-13
AGTACTT235650.027.19298412-13
GAGTACG372050.025.27240412-13
GTACTTT250300.025.21060814-15
GTACGGG374800.023.80238214-15
TACTTTT273300.023.58948314-15
GTATATA26200.023.1346341
Produced by FastQC (version 0.11.5)