FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004923060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3593885
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA109980.30601980864718825Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT102790.28601360366288847Clontech Universal Primer Mix Long (96% over 26bp)
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT67360.1874294809099345No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG46130.1283569173749299Clontech Universal Primer Mix Long (96% over 26bp)
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG45000.12521268766251561No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44760.12454488666164888No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG39710.11049324060174436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT39560.11007586497620264No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC37320.10384305563477964No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)