FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004923073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923630
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26910.29135043253250764No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTT24390.2640667799876574No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTT23480.254214349902017No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGC19570.2118813810725074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19120.20700930026092698No Hit
GTGGTGGCCTGCTCCCAAGGCGTCCGCCCAAGGCCCAACGTGCCCAACCC18690.20235375637430572No Hit
CTCCCAAGGCGTCCGCCCAAGGCCCAACGTGCCCAACCCTTAGAGCCAAT18450.1997553132747962No Hit
ACTTAGAACTGGTACGGACAGGGGGAATCTGACTGTCTAATTAAAACATA18440.19964704481231663No Hit
GTCTAATCTTCTTAGTTTGCTGCAACTAAATGATTCAAGAATCCAACATC16220.17561144614185334No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCA15810.1711724391801912No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACT14880.1611034721695917No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGTTGA14610.15818022368264348No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA13910.15060143130907397No Hit
GGTACGGACAGGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGAT13590.14713684050972792No Hit
CTCTTAGGACCGACTGACCCATGTTCAATTGCTGTTAGCATGGAACCCTT13410.14518800818509578No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTT12910.13977458506111753No Hit
CTCCCGACGTCCCTTGAAAATCCGCGGGAGGGAATAATTCTCACGCCAGG12650.1369596050366489No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTG12550.13587692041185323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12480.13511904117449627No Hit
GAATAATTCTCACGCCAGGTCGTACTCATAACCGCAGCAGGTCTCCAAGG12400.13425289347465977No Hit
TATCTACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTG12350.13371155116226197No Hit
GCCTCTGGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATC12320.13338674577482323No Hit
GTTAACAACCAACTTAGAACTGGTACGGACAGGGGGAATCTGACTGTCTA11950.12938081266307938No Hit
GGCCCAACGTGCCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGA10920.11822916102768426No Hit
GGCTTCGGCCGTCCGGCGTGCGGTTAACAACCAACTTAGAACTGGTACGG10580.11454803330337907No Hit
GTCCTAAGAGAATGGCGAAAGCTATTTCTGAATCAAAGGCGGTGTGTACA10000.10826846247956434No Hit
CTACAATGGCGTGTGCAGAAAGCTTGTTCGAGTTCCGTCCGAGGATGGCT9800.10610309322997304No Hit
CATAAATACACGCCGTGATTTGGGATTGGGGATTGCAATTGTTGCCCATG9360.10133928088087221No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)