FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004923074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923630
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTT24120.2611435315007092No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTT22990.24890919524051838No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGC18070.19564111170057275No Hit
CTCCCAAGGCGTCCGCCCAAGGCCCAACGTGCCCAACCCTTAGAGCCAAT17230.18654656085228932No Hit
GTGGTGGCCTGCTCCCAAGGCGTCCGCCCAAGGCCCAACGTGCCCAACCC17190.18611348700237107No Hit
ACTTAGAACTGGTACGGACAGGGGGAATCTGACTGTCTAATTAAAACATA17060.18470599699013673No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCA16910.1830819700529433No Hit
GTCTAATCTTCTTAGTTTGCTGCAACTAAATGATTCAAGAATCCAACATC15920.17236339226746641No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA15820.17128070764267075No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACT15120.16370191526910127No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGTTGA14690.15904637138247998No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTT13430.1454045451100549No Hit
GAATAATTCTCACGCCAGGTCGTACTCATAACCGCAGCAGGTCTCCAAGG13040.14118207507335187No Hit
TATCTACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTG12680.13728441042408757No Hit
CTCCCGACGTCCCTTGAAAATCCGCGGGAGGGAATAATTCTCACGCCAGG12480.13511904117449627No Hit
GGTACGGACAGGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGAT12320.13338674577482323No Hit
GCCTCTGGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATC12050.130463497287875No Hit
CTCTTAGGACCGACTGACCCATGTTCAATTGCTGTTAGCATGGAACCCTT12040.13035522882539544No Hit
GGCCCAACGTGCCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGA11920.12905600727564068No Hit
GTTAACAACCAACTTAGAACTGGTACGGACAGGGGGAATCTGACTGTCTA11520.1247252687764581No Hit
GGCTTCGGCCGTCCGGCGTGCGGTTAACAACCAACTTAGAACTGGTACGG10050.10880980479196214No Hit
GTATTTATGATTGCCGTAATCTTTCGACTACGGTTTCTCTCGTCCCGCTA9880.10696924092980956No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTG9580.10372118705542263No Hit
GGCTTGCGCCGATCTGAACTCAGATCAAGTCTTGATTTAAAAAACGAACA9560.10350465013046349No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)