FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4963907
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402370.8105913346080013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175770.35409607794827747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169770.342008824903448No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99600.078.042781
GTATCAA271250.075.650391
TATCAAC374050.055.364482
ATCAACG379500.054.5067523
TCAACGC384650.053.7460754
CAACGCA392900.052.615415
AACGCAG397300.052.11726
ACGCAGA418950.049.3672147
CGCAGAG428800.048.231268
GCAGAGT451100.045.846969
AGAGTAC440900.035.61528410-11
CAGAGTA445350.032.08432810-11
AGTACGG285800.031.79556712-13
AGTACTT223300.029.4535912-13
GAGTACT213150.028.96073312-13
GAGTACG290400.027.93665712-13
TACGGGT55300.027.6907114-15
GTACTTT234750.026.3330314-15
TACGGGC76000.026.13453914-15
GTACGGG303350.025.85727514-15
Produced by FastQC (version 0.11.5)