FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004923095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50757533
Sequences flagged as poor quality0
Sequence length150
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4197900.8270496519206321No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3608900.7110077631235545No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT2814720.5545423178860958No Hit
ACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA2486270.48983271113668986No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1852480.3649665163986595No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA1758240.3463998141911271No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT1665680.3281640973370396No Hit
CCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAGTATAG1546740.3047311223735007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1542730.30394109185724216No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT1512890.2980621615317671No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT1443630.28441689630581535No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT1410730.27793509980085124No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT1294740.25508331935675443No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA1280870.25235072003992No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1174660.2314257471890921No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT1166660.22984962645840176No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC1047130.20630041259097442No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC1031840.20328805184444249No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC1018890.20073670641163743No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC950190.18720176963683402No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC911420.17956349454572584No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA894500.17622999920031576No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT863830.17018754634903158No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT863350.17009297910519017No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC859510.16933644115445878No Hit
TTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACT759440.14962114096443577No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG724160.1426704485420913No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG719270.1417070447454568No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT687530.13545378574644282No Hit
CTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGAT638790.1258512701947118No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT572680.11282660250646934No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAG569430.11218630345962638No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT568210.11194594504819609No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA555940.10952856987749976No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT545910.10755250851139672No Hit
CTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC531140.10464259561235964No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG522670.10297387778874123No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAG512050.1008815775187498No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)