FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12657771
Sequences flagged as poor quality0
Sequence length150
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1868241.4759628689759041No Hit
ACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA1145210.9047485532800363No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG907220.7167296674904294No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT776020.6130779265954488No Hit
CCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAGTATAG621110.49069460965915723No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT587970.46451306474101955No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT549800.434357676402899No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA398070.3144866501376901No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT357200.28219818481468817No Hit
GCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATA305140.24106930043212188No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG304900.24087969358902134No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT300130.2371112575823974No Hit
GGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTT297800.23527049114729598No Hit
CTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGAT277630.21933561604171856No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA274390.2167759236598608No Hit
AGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCAT268790.2123517639875141No Hit
ATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAAT259280.2048385928296538No Hit
GTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGT253120.1999720171900724No Hit
GGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCT246700.19490003413713206No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAAT232390.1835947261172603No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC225410.17808032709708527No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA222430.17572604212858645No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA210280.1661271956966199No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAG208730.16490265150159536No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC206800.16337789647166157No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT190780.15072163969469823No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG190360.15038982771927223No Hit
GTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATG189800.14994741175203755No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT189230.1494970954996737No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT185800.1467872977003613No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC184890.14606837175360496No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT183550.1450097335462934No Hit
CAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATA178520.14103589012631054No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC172320.13613771334621239No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC170140.13441545118804882No Hit
TCTGAGATGTTAGTGTTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC165260.1305601120450038No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC164950.1303152032059989No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC163850.12944617184178794No Hit
TCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTT160900.12711558772867673No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC154950.12241491807680831No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA149380.11801445925984914No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC145200.11471214007584749No Hit
TAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCT144030.11378780671573217No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA142230.11236575539247788No Hit
AAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCA140050.11064349323431431No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG139610.11029588068862993No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC138680.1095611541716152No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT137760.10883432793972968No Hit
GGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATC135770.10726217119902073No Hit
CTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCATTACT132220.10445756997815808No Hit
ATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACC132200.10444176940789969No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT131300.10373074374627254No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT128780.10173987189371651No Hit
AGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATG128240.10131325649674022No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGC108700.083.4518362
GTACATG117900.077.766571
CATGCTA120550.076.146794
TAAGACT130550.069.874769
ACATGCT131900.068.9939353
GGTATCA54000.067.969661
ATCAACG196700.058.0872963
TCAACGC196850.057.896744
CAACGCA198700.057.6860735
TGCTAAG160550.057.31216
AACGCAG202050.056.6583636
CGGGACA23550.056.55744
CGCAGAG201700.056.4704448
CGGGGAC16250.055.824624
CCGCAGT26050.054.9164621
ACGCAGA209400.054.6002277
CCCTGGG21300.053.4038242
GGGGTTA580450.052.7541166
GCAGAGT215200.052.5264479
CCACGAC28600.052.3622069
Produced by FastQC (version 0.11.5)