FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6229719
Sequences flagged as poor quality0
Sequence length150
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180980.2905106955867512No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC128300.2059482939760204No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG114260.18341116188386666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103710.1664762086379819No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA95830.1538271629908187No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC95250.15289614186450465No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA94300.15137119346795577No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA93280.14973388045271385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93060.14938073450824987No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT92350.14824103623293441No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT86540.13891477288140924No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC74250.11918675625658237No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA69220.11111255579906573No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT68940.11066309732429343No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC68130.10936287816513071No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC67510.10836764868527778No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA67240.1079342422988902No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA66090.10608825213464683No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA63370.10172208409400167No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC62840.10087132340961126No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67500.075.604711
GTATCAA136600.067.129651
TCAACGC161150.057.2277764
ATCAACG162250.056.617943
CAACGCA165500.056.4200555
AACGCAG168000.055.4519126
ACGCAGA178850.052.289177
CGCAGAG178300.051.926398
TATCAAC183500.050.140232
GCAGAGT197900.046.0608259
GACCGTT13450.036.3980527
ACGGTAC15450.036.345793
GTACTGG50300.033.8192061
GGTTCAC50900.030.8340266
TACTGGT53450.030.1711462
GCGTACA40900.029.5717832
AGCGTAC40500.029.3838061
AGTACGG139850.027.7471142
GGACCGT30400.027.7079126
GAGTACG140100.027.6714441
Produced by FastQC (version 0.11.5)