FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6062023
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269670.4448514959445056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183170.3021598565363411No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA127170.20978145414492821No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC113740.18762713371427325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113100.1865713805440857No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA104950.1731270237674783No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC95490.15752167222064317No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC92240.1521604256532844No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT82130.13548282479297752No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT75300.12421595892988199No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC74710.1232426864761153No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG73040.1204878305476571No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA71920.11864026249982886No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC60860.1003955280275248No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100300.088.390631
GTATCAA249000.063.9641841
ATCAACG316500.049.857753
CAACGCA321700.049.0703355
TCAACGC327600.048.3137364
AACGCAG332700.047.5194746
TATCAAC338250.046.9156882
ACGCAGA345150.045.5119367
CGCAGAG346250.045.388998
GCAGAGT360900.043.447629
CAGAGTA375750.032.46147510-11
AGAGTAC373500.032.32257510-11
GTACTGG24750.031.2737961
CTCTGCA37850.030.174699
GAGTACT217550.028.82240312-13
TCTATAC51750.028.7450123
AGTACGG226200.027.2073712-13
GAGTACG220300.026.8963612-13
TCTCTGC43550.026.3618958
ATTTAGG68450.026.0951751
Produced by FastQC (version 0.11.5)