FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6062023
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA116070.19147073509948742No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC112270.18520220065149867No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA92870.1531996826801878No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC91820.1514675876353488No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC87450.14425877302016174No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA72300.11926711594462773No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT71520.11798041676846162No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG70150.11572044513852883No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT68490.11298208535335483No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC66780.11016124485175988No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59950.077.496921
GTATCAA147400.056.6216851
CAACGCA177000.046.2781645
ATCAACG177750.046.1502083
ACGCAGA183250.044.6020287
CGCAGAG183800.044.4656228
AACGCAG185200.044.3893626
TCAACGC185750.044.3227044
GCAGAGT193800.042.109839
TATCAAC201650.041.4477232
AGAGTAC209350.030.60960210-11
CAGAGTA210900.030.28607610-11
CTCTGCA40600.029.4478239
TCTCTGC46700.027.0023868
GAGTACG233450.026.7246112-13
AGTACGG239800.026.19029412-13
GTACTGG25900.025.953821
TTCTCTG47000.025.5661537
TACGGGT39250.023.22618714-15
TCTATAC50500.022.7344763
Produced by FastQC (version 0.11.5)