FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7752813
Sequences flagged as poor quality0
Sequence length150
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147160.189814974255151No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCT129450.16697165274075357No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115420.14887499543714003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA93300.056.635715
AACGCAG95200.055.5060966
TCAACGC98100.054.1626244
CGCAGAG102750.051.497618
ATCAACG104250.050.9684073
ACGCAGA104300.050.594257
GTATCAA118900.047.822691
GCAGAGT139450.038.3576359
GGTATCA64700.037.0691681
TATCAAC151650.036.889212
GGGTTAG65400.035.1151477
TAGGGGT66100.034.6368184
ATAGGGG68350.033.4972653
GGGGTTA71200.032.962426
GAGTACG59450.032.862771
GTCTCGC6300.032.399531
CGGGGAC3950.030.9859054
AGTACGG63700.030.9693342
AGGGGTT75050.030.2159185
GTACGGG71950.029.7877561
Produced by FastQC (version 0.11.5)