FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7752813
Sequences flagged as poor quality0
Sequence length150
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCT128180.1653335376462711No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94180.1214784878727244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90140.11626747607610295No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA73850.053.4275
AACGCAG74450.052.9960826
CGCAGAG75300.051.5353138
TCAACGC74500.051.1223144
ACGCAGA79850.049.590227
ATCAACG79900.047.937563
GTATCAA98550.041.1091651
GCAGAGT107450.035.110199
GGGTTAG67350.031.7487897
GAGTACG57950.031.6528421
AGTACGG61300.030.5389982
ATAGGGG71300.030.293043
TAGGGGT69000.030.259384
GGGGTTA71800.030.1835236
TATCAAC131200.029.9640662
GTACGGG69300.027.8234141
GGTATCA58550.027.7515981
AGGGGTT75400.027.405725
ACGGGAC7450.027.0590693
GTCTCGC6200.026.7897431
Produced by FastQC (version 0.11.5)