FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004923146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489021
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC18730.3830101365790017No Hit
GTATTTCACTGTCTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGG12520.25602172503839304No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT12460.25479478386408766No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT9470.1936522153445353No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT8490.1736121761642138No Hit
GCCTTTTTCTCTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGA8150.16665950950981656No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT7120.14559701935090721No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA6860.1402802742622505No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC6460.13210066643354784No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC6400.13087372525924246No Hit
GTATCAACGCAGAGTACGGGTCAACGTTAGAACATCAAACATTAAAGGGT6080.12433003899628033No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG5950.12167166645195196No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT5930.12126268606051684No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG5730.11717288214616552No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG5680.11615043116757766No Hit
TTTTATGACTGTCTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGG5050.103267548837371No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)