FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6869900
Sequences flagged as poor quality0
Sequence length150
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148300.21586922662629732No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCT141050.20531594346351476No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101050.1470909329102316No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA95190.13856096886417563No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT77950.11346598931571057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70210.10219944977365027No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATATAG68930.10033624943594521No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA109750.057.7303545
TCAACGC109300.056.9124
GTATCAA112800.056.805561
AACGCAG112250.056.444196
ATCAACG112550.055.4605143
CGCAGAG118100.053.3418968
ACGCAGA121000.052.3605777
GTCTCGC11950.044.734251
GGTATCA48600.042.3628271
GCAGAGT150200.040.9832539
TATCAAC160450.040.0737462
GAGTACG95550.033.7194481
TCTGTCG18450.031.9983658
GGGTTAG80300.031.2908237
ACGGGAC15450.031.2217063
AGTACGG103700.031.1756692
CGCTCTG16050.030.9528125
ATAGGGG84900.030.3588713
TAGGGGT85700.030.1594834
TACGGGA52950.029.3721682
Produced by FastQC (version 0.11.5)