FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004923153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9465474
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT923930.976105369894841No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG599520.6333755710490568No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA540020.5705155388943016No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT511190.5400574762552832No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT449430.47480981934977584No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA284310.30036530658686506No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT266920.2819932736596181No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT249420.26350502890821953No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG244970.25880373238572096No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT217470.22975077634780888No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG205200.21678787559925683No Hit
GCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATA204230.21576309860446502No Hit
GGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTT204130.21565745149159987No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC198780.2100053309533152No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAG196510.20760714149127663No Hit
GGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCT181420.19166499215992774No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT180520.19071416814414155No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC171040.18069882184452676No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAAT166820.17624051368161806No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT165790.17515234841910715No Hit
AGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCAT149480.15792130431080365No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG148680.15707612740788257No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA142800.15086407717141265No Hit
AGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATG142770.1508323830375531No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC138000.14579301575388617No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC136710.14443016799792593No Hit
CTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTT136610.1443245208850608No Hit
CAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATA131990.13944362427069157No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA130830.138218117761456No Hit
TCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTT128590.13585162243327698No Hit
ATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACC127620.13482684543848517No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG125240.13231244415229496No Hit
AAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCA125090.13215397348299726No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT124470.13149896138323341No Hit
ATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAAT123100.13005159593698107No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT122820.1297557840209587No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC121540.12840350097628495No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG119850.1266180647688642No Hit
AAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTA117610.12425156944068518No Hit
GTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGT115650.12218088602852853No Hit
CCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATA115420.1219378976689387No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA113690.12011020261637188No Hit
AATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTAC112870.11924389629087778No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC106670.11269377529323941No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA105140.11107737446640284No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC102660.1084573260673475No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC101750.10749593734027477No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATCT99180.10478080653964081No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA98780.10435821808818027No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG97020.1024988289017539No Hit
TCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGG96920.10239318178888876No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT96120.10154800488596767No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)