FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004923186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3871444
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA151350.39093940142231165No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC118880.30706888695794127No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC116000.29962980221333435No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA105070.27139744240133656No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCG105070.27139744240133656No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG101990.2634417545494652No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCA99730.25760413943737787No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT99660.2574233283498354No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC96760.249932583294502No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT95060.24554145688275483No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTT88790.22934594947001688No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA88070.22748617828386516No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC83430.21550098619533176No Hit
CTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAG81170.20966337108324437No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT80430.2077519395863662No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAG78150.20186266416355242No Hit
GTATCAACGCAGAGTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTT77180.1993571390933202No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGA76640.19796231070370643No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTG72030.1860546090812627No Hit
CCTCAAGGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCA71930.18579630752763052No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA67460.17425022808027185No Hit
GGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGT65930.17029821430969944No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC64390.1663203703837638No Hit
CCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAGGG63040.1628332994097293No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGT62580.16164511226302125No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA59870.15464514015958902No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG59380.15337946254679133No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCG58740.15172633260354534No Hit
GTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACAAACCGCCTG58730.15170050244818212No Hit
GTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTA58210.15035733436929477No Hit
CTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCC57400.14826509178487407No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT56500.14594037780218438No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC56410.14570790640391545No Hit
GTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACC56080.14485551127692922No Hit
ACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAGGGCACAACCTCCAAG56070.14482968112156602No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACG55790.1441064367713959No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC55210.14260828776032922No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT55040.1421691751191545No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA54830.1416267418565269No Hit
CTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCG53740.1388112549219361No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCG52140.13467843006382116No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGC52130.13465259990845793No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC52030.13439429835482575No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG51710.13356773338320274No Hit
CTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTC51420.13281865887766942No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA51280.13245703670258435No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT51220.13230205577040505No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGG50420.13023564334134757No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAAT50350.13005483225380504No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA50050.1292799275929085No Hit
CTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCG49850.12876332448564412No Hit
GGTTGAGCCCGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGC49520.12791092935865792No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC49160.12698104376558203No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG48930.126386950192228No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG48490.1252504233562464No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA48480.12522459320088317No Hit
GGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTA48010.12401057589881191No Hit
GTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATT47780.12341648232545789No Hit
ATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAAC47750.12333899185936824No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGC47700.12320984108255215No Hit
GTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG47550.12282238875210387No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC47500.12269323797528778No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC47380.12238327611092915No Hit
GATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGAC46940.12124674927494754No Hit
CCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATAC46700.12062682554623029No Hit
GTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCC46520.12016188274969236No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC46100.11907701622443718No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC45870.11848292265108316No Hit
CCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGT45850.11843126234035674No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGG45170.11667481177565786No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT45120.11654566099884177No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA44830.11579658649330843No Hit
GGTATCAACGCAGAGTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCT44580.11515083260922798No Hit
GAGTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACT44540.11504751198777509No Hit
TATCAACGCAGAGTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTG44460.11484087074486937No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC44460.11484087074486937No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC44290.11440175810369464No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC43900.11339438204452912No Hit
TCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGA43860.11329106142307624No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAA43720.11292943924799119No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC43440.11220619489782106No Hit
CGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGC43440.11220619489782106No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA43390.11207704412100497No Hit
CCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAG43260.11174125210128313No Hit
GTATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTA43040.11117298868329234No Hit
CTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTG43010.11109549821720269No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGG42570.10995897138122107No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT42240.10910657625423485No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGC41890.10820252081652222No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAA41880.108176690661159No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC41270.10660105118400266No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT40940.10574865605701646No Hit
AGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCA40880.10559367512483714No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTC40560.10476711015321415No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC40270.1040180356476808No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC39950.10319147067605783No Hit
CCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGT39880.10301065958851528No Hit
CCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTGCCCTTG39810.10282984850097276No Hit
GTCTCGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAG39780.10275235803488311No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAA39310.10153834073281184No Hit
GTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAACGCGTTAGC39110.10102173762554748No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCG39100.10099590747018425No Hit
GGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGA38750.1000918520324716No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)