FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004923219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7991011
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGG803921.0060304009092216No Hit
TCATAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGG443450.5549360400079539No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC382930.479200942158633No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA377450.47234323666930256No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC333700.4175942193046662No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG332390.4159548772990051No Hit
TGAAATACAGAGAAAAAGGCCTGTCTCTTATACACATCTCCGAGCCCACG305870.38276758723020154No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACCTGTCTCTTATACACATCTC303530.379839296930013No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA265070.3317102178935807No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC246940.3090222250976754No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT246480.3084465782865272No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA210530.263458528589186No Hit
TGAAATACAGAGAAAAAGGCTGTCTCTTATACACATCTCCGAGCCCACGA202730.2536975609218909No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC196830.2463142648658599No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA194670.24361122766568588No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA192870.24135869666554083No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA178270.22308816744214216No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC169670.21232607488589367No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA156610.19598271107373022No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC151010.18897483685105676No Hit
TGAAATACAGAGAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGAC143890.18006482533937196No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA130270.16302067410494117No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG127160.15912880109913502No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA127000.15892857612134434No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACAACCCCCCGTACTCTGCGTT125730.15733929036013092No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC122270.15300942521540767No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT119710.14980582557075695No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT116300.14553853073159329No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG115930.14507551047045236No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC107810.13491409284757586No Hit
TGAAATACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGAGA107170.1341131929364132No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA102190.12788119050267857No Hit
TGAAATACAGAGAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACC99710.12477770334692317No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG99660.12471513304136361No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA99550.12457747836913252No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACAACCCCACGTACTGCAGTCA99500.12451490806357293No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT98840.12368898003018641No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA96220.1204102960188642No Hit
TCATAAAAAGGTCAATCCAACCACTGTCTCTTATACACATCTCCGAGCCC95640.11968448047437302No Hit
TCATAAAAAGGTCAATCCCTGTCTCTTATACACATCTCCGAGCCCACGAG95040.118933636807658No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT91310.11426589201291301No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT91210.11414075140179383No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG90030.11266409219058765No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA88210.11038653306821877No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA87750.1098108862570706No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA86620.10839679735142399No Hit
TCATAAAAAGGTCAATCCAACCACCCAAATCAAGCGTTACGTACTGCAGT85350.10680751159021055No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG83030.10390424941224584No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT82360.10306580731774741No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG81290.1017268027787723No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)