FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7991011
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTCACTGTCTCTTATACACATCTGACGCTGCCGACGATCTACTCTG763060.9548979472059291No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC352800.44149607602842744No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA338320.42337571553837183No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG332540.4161425882156839No Hit
TTTTATGACTGTCTCTTATACACATCTGACGCTGCCGACGATCTACTCTG328460.4110368512820217No Hit
GTTTTGGGATGCCTTTTTCTCTGTATTTCACTGTCTCTTATACACATCTG288850.361468655217719No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC286090.35801477435082996No Hit
GCCTTTTTCTCTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGA285500.35727644474522685No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT259950.32530301860427924No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA238820.2988608074747989No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC214100.26792604840614037No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC204730.25620037314427424No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA202510.2534222515774287No Hit
CCTTTTTCTCTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGAC191080.2391186797265077No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC179660.22482762193669864No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA175250.21930892098634328No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA174910.21888344290853812No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA161590.20221471350746484No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA158220.19799747491274886No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC144740.1811285205338849No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG140660.1760227836002228No Hit
TTTTCTCTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGACGAT131540.16460995986615462No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA129860.16250759759935257No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC121420.15194573002089473No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA118390.1481539695039839No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT103730.12980835591391376No Hit
CTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGACGATCTACTC101260.1267173828192703No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT101070.1264796156581439No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA100760.12609167976367447No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT100660.1259665391525553No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG98950.1238266347024175No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT94050.11769474475757824No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC93660.11720669637421348No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA92740.11605540275191713No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA90790.11361516083509333No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA90580.11335236555174306No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA89990.11261403594613997No Hit
TTTCTCTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGACGATC89950.11256397970169231No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG89420.11190073446276072No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA89390.11186319227942498No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA88930.1112875454682768No Hit
TGGTTGGATTGACCTTTTTATGACTGTCTCTTATACACATCTGACGCTGC88900.11125000328494104No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT87040.1089223879181245No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG86910.10875970512366959No Hit
GGATTGACCTTTTTATGACTGTCTCTTATACACATCTGACGCTGCCGACG86800.10862205045143851No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT85840.10742070058469448No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG84570.10583141482348103No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT82410.10312837762330698No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA290550.059.993191
GGTATCA148500.055.124821
ATCAACG405850.042.4803433
TCAACGC417650.041.337114
TATCAAC428600.040.6696742
CAACGCA423600.040.6441275
AACGCAG427150.040.5431066
TATAAGA92800.040.515792
CGCAGAG431150.040.0013858
ACGCAGA431950.039.9961667
GTATAAG94350.039.9132421
GCAGAGT444700.038.7959259
GACAGTC48850.037.3877269
TAAGAGA97050.036.596034
TCTCTGC61100.036.4154438
ATAAGAG100000.036.1113663
GTCTATA37700.035.347762
TACGTCC46300.034.4357074
AAGAGAC97050.033.6536375
ACGTCCA46350.033.6284455
Produced by FastQC (version 0.11.5)