FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7071093
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA258650.069.402081
GGTATCA123450.053.5466881
CAACGCA368150.048.021775
TATCAAC372400.047.898212
TCAACGC375700.047.096084
ATCAACG382600.046.262943
AACGCAG388750.045.6282586
ACGCAGA398950.044.345587
CGCAGAG412250.042.899268
GCAGAGT433150.040.7481049
AGAGTAC411900.033.64715610-11
AGTACGG470400.029.2231412-13
CAGAGTA428000.028.24780310-11
TACGGGG139050.025.72930314-15
TACGGGT94800.025.25346414-15
GAGTACG480800.024.97308312-13
TACGGGC140300.024.1858514-15
GTACGGG483550.023.97978214-15
TACGGGA178600.022.19637714-15
ACGGGAT35250.019.49251416-17
Produced by FastQC (version 0.11.5)