FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9603833
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTAC1449571.5093661041377957No Hit
TCATAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTAC673100.7008659979822639No Hit
TGAAATACAGAGAAAAAGGCCTGTCTCTTATACACATCTCCGAGCCCACG567110.5905038123840762No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC424720.44224009309616275No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC401270.41782275889220477No Hit
TGAAATACAGAGAAAAAGGCTGTCTCTTATACACATCTCCGAGCCCACGA370290.38556480521891623No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG345390.3596376571729225No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACCTGTCTCTTATACACATCTC301970.31442654198589254No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA288700.3006091422039513No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT285240.29700641400157624No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA278620.29011333287448876No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA225130.23441682086725166No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC224690.2339586704600132No Hit
TCATAAAAAGGTCAATCCAACCACCCAAATCAAGCGTTACGTACTGCAGT207450.21600750450367057No Hit
TGAAATACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCT198050.20621974580357655No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA197510.20565747030378392No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC189880.1977127257418991No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA185750.19341235941941098No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA185250.19289173395664003No Hit
TGAAATACAGAGAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGAC181200.18867466770819527No Hit
TGAAATACAGAGAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACC158600.16514239679094794No Hit
TCATAAAAAGGTCAATCCAACCACTGTCTCTTATACACATCTCCGAGCCC155090.1614876060422958No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA150120.1563125889423525No Hit
TGAAATACAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC142640.14852403201929895No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT141370.14720164334386074No Hit
TCATAAAAAGGTCAATCCCTGTCTCTTATACACATCTCCGAGCCCACGAG138620.14433820329862046No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC128140.13342589359894116No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACAACCCCCCGTACTCTGCGTT127170.13241588020116551No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT124340.12946914008188187No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC119210.12412752283385187No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA118580.12347153475076045No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT111450.11604741565164658No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG109480.113996151328329No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG109160.1136629510321556No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT108410.11288201283799915No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG106650.11104941120904539No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC105850.11021641046861186No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC100040.10416674259121333No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT99420.10352116701737733No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA99400.1035003419988665No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT98270.10232372845300412No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGAG461050.093.921136
ATACAGA475450.091.464155
AATACAG506400.090.28814
ACAGAGA482400.089.493267
GAAATAC721350.088.7092742
CAGAGAA477350.086.7910468
TGAAATA741000.085.982841
TCATAAA356650.082.52291
AGAGAAA517700.079.693479
AGGTCAA225050.079.326399
AAGGTCA226650.078.792638
AAATACA586250.078.771253
AAAGGTC242500.077.368947
TAACAAG89500.076.253586
AATACCT240050.075.335364
TAAAAAG277000.073.870754
TCATGTA63650.072.63621
CATGTAA64750.071.1546552
GTAACAA97700.069.7318955
AACAAGC101900.066.682747
Produced by FastQC (version 0.11.5)