FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004923248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9603833
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTCACTGTCTCTTATACACATCTGACGCTGCCGACGATCTACTCTG1381751.438748466367543No Hit
GCCTTTTTCTCTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGA528630.5504364767692232No Hit
TTTTATGACTGTCTCTTATACACATCTGACGCTGCCGACGATCTACTCTG501460.5221456891222495No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT350520.3649792744209525No Hit
CCTTTTTCTCTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGAC346450.36074138315399695No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG326250.3397081144580502No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC325730.3391666639767684No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC316220.3292643676748648No Hit
GTTTTGGGATGCCTTTTTCTCTGTATTTCACTGTCTCTTATACACATCTG288430.300328004454055No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA235850.245579030789061No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC217290.22625341361100304No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA215410.22429586187098421No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA200250.20851049783976874No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA199650.20788574728444362No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA197870.206032320636979No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC195880.2039602312951506No Hit
CTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGACGATCTACTC187060.1947763981318709No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA169130.17610676903690434No Hit
TTTTCTCTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGACGAT166500.1733682791027291No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA145540.15154365970337053No Hit
TTTCTCTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGACGATC143850.14978394563920466No Hit
TGGTTGGATTGACCTTTTTATGACTGTCTCTTATACACATCTGACGCTGC142430.14830536932493515No Hit
TCTCTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGACGATCTA132230.13768460988440762No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC130920.13632057117194768No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT129510.1348524073669336No Hit
GGATTGACCTTTTTATGACTGTCTCTTATACACATCTGACGCTGCCGACG127730.132998980719469No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG124460.12959409019294693No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT117510.12235739626043061No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA109240.11374625110619895No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA107160.11158044918107175No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG106780.11118477382936584No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT105410.1097582600613734No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT105360.1097061975150963No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA102100.10631171949782967No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT101620.10581191905356954No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA96830.10082432712022378No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTACACTCAATTGAT96440.10041823925926241No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)