FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4838244
Sequences flagged as poor quality0
Sequence length125
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC165510.34208692244541616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117790.24345609688143055No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT111270.22998013328802766No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT87970.18182216523184858No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT86370.17851518030095218No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT70260.1452179757779889No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC61110.126306155704425No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA57080.11797668740972965No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC56750.11729462176773225No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG52180.10784904605885937No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG50550.10448005516050865No Hit
GTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACT50040.10342595371378542No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG48750.10075969711325018No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG48730.10071835980161396No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA312750.080.301181
TATCAAC438800.057.5277672
GGTATCA109050.056.5206531
ATCAACG477500.052.922943
TCAACGC485650.051.9072464
AACGCAG495300.051.1223876
ACGCAGA506350.049.907077
CAACGCA507750.049.758425
CGCAGAG511300.049.4458478
GCAGAGT521000.047.7717639
GTTATCA32750.047.6067621
TTATCAA32150.046.5763472
AGAGTAC516900.032.5760310-11
CAGAGTA521200.031.29044210-11
GAGTACT75700.027.92975612-13
GTTCGGA6700.027.6232191
AGTACGG539800.027.16817712-13
GAGTACG543300.026.08994512-13
ACTGTGC96450.024.7120298
TACGGGC134700.024.6060314-15
Produced by FastQC (version 0.11.5)