FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4686167
Sequences flagged as poor quality0
Sequence length150
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158470.3381654985833838No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA106580.22743534321333406No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC98250.20965962160546134No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC93430.19937402999082193No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT89830.19169184538237752No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88310.1884482563254788No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT78200.16687412121676415No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA74300.15855175455761608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70460.1503574243086087No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC67320.14365685217791No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC62050.13241098748721503No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG54230.11572357536553861No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC50650.10808406956047448No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA49690.10603548699822263No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA247250.078.158491
GGTATCA138800.060.9051931
CAACGCA362750.053.6659365
TATCAAC372450.052.132412
ACGCAGA374550.051.745117
CGCAGAG375500.051.4591648
AACGCAG378400.051.3697476
ATCAACG378250.051.0872233
GCAGAGT382450.050.1099059
TCAACGC403950.047.9067234
ATTTAGG47950.037.203951
CTATTGA53700.034.052559
TAGGCAT31850.033.6803255
GCTATTG58600.031.205158
ACTGTGC33750.031.1436318
TTCTCTG27900.030.4496217
TTTAGGG59350.030.3259262
TAGTACT29450.029.3353184
TCTCTGC28850.028.4478328
TATTCTC30350.028.2284955
Produced by FastQC (version 0.11.5)