FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004923269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2165525
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311191.437018736795927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188240.8692580321169232No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175530.8105655672412001No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT69440.32066127151614504Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66450.30685399614412207No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT38330.1770009581972039No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGC38030.17561561284215144No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33970.15686727237044135No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA31700.14638482585054433Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTCTAAGGCGCTTGAGAGAACTCAGGAGAAGGAACTCGGCAAATTGACA27020.12477343831172578No Hit
ATATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27010.12472726013322405No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAG24440.11285946825827455No Hit
AGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23390.1080107595155909No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT23160.10694866141005069No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACA23130.10681012687454544No Hit
ATATACGACACTCTTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA22160.10233084355987578No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTAATAAACA21830.10080696366931807No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)