FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5350461
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226900.424075607690627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114910.21476654067752293No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110080.20573928115726853No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA73030.136492911545379No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC54010.10094457281344543No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76000.067.0965041
GTATCAA182150.066.98631
ATCAACG261900.048.898253
TCAACGC264400.048.4129684
TATCAAC264800.048.295412
AACGCAG269650.047.510916
CAACGCA270350.047.347035
ACGCAGA293850.043.55737
CGCAGAG303450.042.1793148
GCAGAGT326500.039.2379729
AGAGTAC312850.032.9157210-11
AGTACGG210000.029.79545412-13
CAGAGTA323500.027.95640810-11
TACGGGT46050.025.31106814-15
GAGTACG211500.025.21535312-13
TTAGGGC49500.024.851163
ATACGGG13650.023.947281
GGGCTAT45750.023.508916
TACGGGA87200.023.39169514-15
GAGTACT158900.023.3346712-13
Produced by FastQC (version 0.11.5)