FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5350461
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA73690.13772645011336407No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA139500.067.220151
GGTATCA59000.053.4492231
ATCAACG182300.050.7496153
AACGCAG183900.050.2380566
TCAACGC184450.050.0608254
CAACGCA189050.048.802125
ACGCAGA191000.048.1905757
TATCAAC195550.047.829962
CGCAGAG197700.046.5870558
GCAGAGT218400.042.1498269
AGAGTAC205000.034.3048310-11
AGTACGG229800.030.29181312-13
CAGAGTA212500.028.88209510-11
TACGGGT49200.026.233714-15
GAGTACG232200.026.18815812-13
TACGGGA93750.024.01372314-15
GGGCTAT44600.023.594026
GTACGGG251800.023.56253814-15
TACGGGC57000.022.80034814-15
TTAGGGC49200.022.734313
Produced by FastQC (version 0.11.5)