FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004923291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008106
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGTGGTTGTTTCCCTTGCATGGGACGAAAGCTTGGCTCCAAAG65810.6528083356313721No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT42100.41761481431516134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38610.38299543897169547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30530.30284513731690915No Hit
TCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAA23420.23231683969741276No Hit
TTGCAGGAGAACGTCGCTATGGAACCTGCATCTACCAGGGAAGACTCTGG18480.18331405625995678No Hit
CTCCTGCAATGCACGCTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTT16690.16555798695772073No Hit
CTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGCAGATTGCAGC16330.1619869339136956No Hit
CTATTGCAGAATACCAGCGTGCATTGCAGGAGAACGTCGCTATGGAACCT16230.1609949747347997No Hit
GAATACCAGCGTGCATTGCAGGAGAACGTCGCTATGGAACCTGCATCTAC15200.15077779519217224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14980.14859548499860134No Hit
GTACATGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT13660.13550162383717584No Hit
GAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTT13530.13421207690461123No Hit
CTTCTGGGATGTCCGCTGCAATCTGCTCCGGGGCTGCAGCAACCTCATCA13270.13163298303948195No Hit
CCCTTGCATGGGACGAAAGCTTGGCTCCAAAGCATCCAGGCTCAAGGAAA13160.1305418279426965No Hit
ATACCAGCGTGCATTGCAGGAGAACGTCGCTATGGAACCTGCATCTACCA12600.12498685654087963No Hit
GCCCAGAGTCTTCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGC12250.12151499941474408No Hit
ACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCT12080.11982866881062111No Hit
GGACGAAAGCTTGGCTCCAAAGCATCCAGGCTCAAGGAAAAACATGGCCT11220.11129781987211662No Hit
CTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCTTT11200.11109942803633746No Hit
GCTCCAAAGCATCCAGGCTCAAGGAAAAACATGGCCTGCTATTGCAGAAT11090.110008272939552No Hit
GGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTGGTATT10990.10901631376065612No Hit
TCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTG10840.10752837499231231No Hit
CTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCC10700.1061396321418581No Hit
CTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCTTTGGAGCCAA10470.10385812603039758No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)