FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004923292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008106
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGTGGTTGTTTCCCTTGCATGGGACGAAAGCTTGGCTCCAAAG56990.5653175360527564No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT44170.4381483693183058No Hit
TCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAA22480.2229924234157916No Hit
TTGCAGGAGAACGTCGCTATGGAACCTGCATCTACCAGGGAAGACTCTGG20530.20364921942732211No Hit
CTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGCAGATTGCAGC16560.1642684400251561No Hit
TAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTT15380.1525633217141848No Hit
GAATACCAGCGTGCATTGCAGGAGAACGTCGCTATGGAACCTGCATCTAC15060.14938905234171804No Hit
CTATTGCAGAATACCAGCGTGCATTGCAGGAGAACGTCGCTATGGAACCT14770.14651237072292No Hit
CTCCTGCAATGCACGCTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTT14760.1464131748050304No Hit
GAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTT14740.14621478296925125No Hit
GTACATGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT14030.13917187279909057No Hit
GGACGAAAGCTTGGCTCCAAAGCATCCAGGCTCAAGGAAAAACATGGCCT12830.12726836265234012No Hit
CTTCTGGGATGTCCGCTGCAATCTGCTCCGGGGCTGCAGCAACCTCATCA12140.12042384431795862No Hit
CCCTTGCATGGGACGAAAGCTTGGCTCCAAAGCATCCAGGCTCAAGGAAA11820.11724957494549185No Hit
GCCCAGAGTCTTCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGC11740.11645600760237515No Hit
ACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCT11290.11199219129734374No Hit
CTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCTTT11070.10980988110377281No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11020.10931390151432488No Hit
GGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTGGTATT10900.10812355049964985No Hit
ATACCAGCGTGCATTGCAGGAGAACGTCGCTATGGAACCTGCATCTACCA10380.1029653627693913No Hit
GCTCCAAAGCATCCAGGCTCAAGGAAAAACATGGCCTGCTATTGCAGAAT10330.10246938317994338No Hit
AGGTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAG10290.10207259950838503No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)