FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4685871
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT168990.3606373286844644No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT137840.294160893460362No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130920.27939309468826606No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG112690.24048890803865494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97880.20888325777640915No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA97340.20773085729419355No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT86430.1844480994035047No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT82410.17586911803589983No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA80110.17096074561164828No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC74540.15907394804509128No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA72610.15495518335865413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69570.14846759545877383No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC62200.13273946295149824No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC60700.1295383505008994No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCG54680.11669121919916277No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA52790.11265781751140823No Hit
TTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATT52250.11150541702919264No Hit
GTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAAC51840.11063044629269564No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG51750.11043837954565972No Hit
CCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATA50780.1083683268276058No Hit
GACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTAAGTTA50700.10819760083024053No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG48530.10356665815170756No Hit
GTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATAT48070.10258498366685724No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA47180.10068565694616859No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG4850.089.6347661
GGTATCA66200.087.078861
GTATCAA159000.058.5780561
ATCAACG229750.044.783173
CAACGCA235000.043.858665
TCAACGC236350.043.6333244
AACGCAG238750.043.2445566
ATACGGG16600.043.0495381
TATCAAC241700.042.495162
ACGCAGA252100.040.9060177
TCTATAC64650.040.7765583
CGCAGAG253750.040.603148
GCAGAGT260250.039.6118939
CTCTGCA21800.037.929419
TACCAAC66100.035.559627
AGCGTAA2250.034.407741
TTCTCTG23900.033.363257
AGAGTAC268650.031.63087510-11
GTCACTC20300.031.3549069
TCTCTGC26250.031.2728568
Produced by FastQC (version 0.11.5)