FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905637
Sequences flagged as poor quality0
Sequence length125
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238140.4854415440848966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77510.15800190678600964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71420.14558761685791263No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA263150.076.435751
GGTATCA86400.058.5411641
TATCAAC366350.055.2253952
ATCAACG367100.055.0333983
TCAACGC372500.054.355464
CAACGCA379500.053.500615
AACGCAG381000.053.363566
ACGCAGA397950.051.104727
CGCAGAG404600.050.389498
GCAGAGT422300.047.5073979
GTTATCA36200.034.078671
AGAGTAC414350.033.3438210-11
CAGAGTA420550.031.25896310-11
AGTACGG326150.028.86524812-13
GAGTACT151050.028.39766912-13
GAGTACG328050.026.94610212-13
AGTACTT161650.026.93442512-13
TACGGGT71500.025.40861114-15
TTATCAA46550.025.296952
GTACTTT171250.024.85153214-15
Produced by FastQC (version 0.11.5)