FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4315884
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284950.6602355392313602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151310.3505886627166068No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT83050.19242871217113341No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80430.18635811342473524No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT78850.182697217997518No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG77550.1796850888485418No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC70790.16402201727386556No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG65540.1518576495568463No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA58750.13612506730950136No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA58340.13517508811636272No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA57660.13359951286920593No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA55960.12966057475131398No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA54970.1273667225532475No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG52800.12233878389687954No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT52130.12078637887394564No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC49970.11578161044180056No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA49080.113719460485963No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTT46660.1081122662240227No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA45830.10618913761352251No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT43710.10127705007826901No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC43510.10081364559381115No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83400.081.310381
GTATCAA218950.070.42021
GCGCAGA13350.063.2723961
ATCAACG277850.055.8580743
TCAACGC286700.054.46584
CAACGCA289050.053.981835
GCTCTAG4250.053.1865541
AACGCAG300150.052.025746
GCGTACT2900.051.280141
TATCAAC325050.047.3066942
ACGCAGA343400.045.4554337
GCGTAAG1600.044.6137241
CGCAGAG354100.044.065098
GTCTTAC4350.043.7590521
GCAGAGT376950.041.5828369
GATCTAC5000.040.4497761
CGTACTT3100.038.377842
GTATAAA10650.036.3053781
GCGTATA1754.474714E-1033.9914051
GCATAGA4400.033.7982751
Produced by FastQC (version 0.11.5)