FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4315884
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG100180.2321193062649506No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT75390.17468032041639672No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT71570.16582929476325128No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66840.1548697787058225No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG64000.14828943502652064No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC62010.14367856040616475No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA61230.14187128291677903No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA55010.12745940345013906No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA53350.12361314622913869No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA52410.12143514515218666No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA51670.11972054855969252No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG51030.1182376542094273No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT49150.11388165205552328No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG48790.1130475239834991No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT46840.10852933026003479No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT46450.10762569151534192No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA46020.1066293718737575No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT45230.10479892416014888No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC45160.10463673259058863No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA44010.10197215680495583No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA133150.062.5377661
GGTATCA56950.060.2611771
ATCAACG176500.046.6708263
TCAACGC178500.046.144694
CAACGCA180350.045.703265
AACGCAG189250.043.611736
ACGCAGA218900.037.699717
CGCAGAG220950.037.375978
TATCAAC228700.036.2784272
GCAGAGT233500.035.697719
GCTCTAG4600.032.3249361
TAGATGG6800.030.6022597
TCCAGTA13150.030.3032682
TACATGG318650.028.5200212
CATGGGT49500.027.8735944
ACATGGG317550.027.7759843
GTACATG363950.027.7165811
GTACACT13150.027.131596
AGAGTAC236400.026.92253110-11
CATGGGA126200.026.9083424
Produced by FastQC (version 0.11.5)