FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004923441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3781
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.7934408886537953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.7140967997884158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.449616503570484No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG100.26448029621793173No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC100.26448029621793173No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG90.23803226659613858No Hit
GTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACC90.23803226659613858No Hit
GATCGGAAGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC90.23803226659613858RNA PCR Primer, Index 26 (100% over 26bp)
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG90.23803226659613858No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT80.21158423697434542No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG80.21158423697434542No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA80.21158423697434542No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGT70.18513620735255223No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT70.18513620735255223No Hit
GATCGGAAGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC70.18513620735255223RNA PCR Primer, Index 1 (96% over 25bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT70.18513620735255223No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC70.18513620735255223No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC60.15868817773075905No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG60.15868817773075905No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG60.15868817773075905No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGC60.15868817773075905No Hit
GCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAG60.15868817773075905No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA60.15868817773075905Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAA50.13224014810896587No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA50.13224014810896587No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCACCGTGTAC50.13224014810896587No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGC50.13224014810896587No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA50.13224014810896587No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT50.13224014810896587No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA50.13224014810896587No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCT50.13224014810896587No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC50.13224014810896587No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG50.13224014810896587No Hit
GTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATGCA50.13224014810896587No Hit
CTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATACAC50.13224014810896587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG50.13224014810896587No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC50.13224014810896587No Hit
GTATCAACGCAGAGTACATGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG40.10579211848717271No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC40.10579211848717271No Hit
GAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAA40.10579211848717271RNA PCR Primer, Index 26 (96% over 31bp)
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCA40.10579211848717271No Hit
CTTCACGACGGACGTTAGCACCCGCCGTGTGTCTCCCGTGATAACATTCT40.10579211848717271No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTT40.10579211848717271No Hit
TTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTT40.10579211848717271No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT40.10579211848717271No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTG40.10579211848717271No Hit
TTTTTCCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTA40.10579211848717271No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC40.10579211848717271No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC40.10579211848717271No Hit
GGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTA40.10579211848717271No Hit
GATCGGAAGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGC40.10579211848717271RNA PCR Primer, Index 17 (96% over 25bp)
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG40.10579211848717271No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA40.10579211848717271No Hit
GGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATA40.10579211848717271No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGCGAAC40.10579211848717271No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC40.10579211848717271No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC40.10579211848717271No Hit
GTACATGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTT40.10579211848717271No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTC40.10579211848717271No Hit
GAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCA40.10579211848717271No Hit
CTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCG40.10579211848717271No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACG40.10579211848717271No Hit
CGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAA40.10579211848717271TruSeq Adapter, Index 8 (100% over 48bp)
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC40.10579211848717271No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTC40.10579211848717271No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA40.10579211848717271No Hit
GAAGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAA40.10579211848717271RNA PCR Primer, Index 26 (96% over 31bp)
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA40.10579211848717271No Hit
ATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAAC40.10579211848717271No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATC40.10579211848717271No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA40.10579211848717271No Hit
GAAGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAA40.10579211848717271TruSeq Adapter, Index 12 (96% over 27bp)
CACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTG40.10579211848717271No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG40.10579211848717271No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC40.10579211848717271No Hit
GTACATGGGAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG40.10579211848717271No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCCGATTAAC40.10579211848717271No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)