FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5288343
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68960.13040001376612675No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA204400.065.206831
GGTATCA96650.053.314091
CAACGCA280100.046.9798365
TCAACGC285100.046.0998734
AACGCAG287300.045.751416
ATCAACG287400.045.710683
TATCAAC291650.045.5762862
ACGCAGA293550.044.7216457
CGCAGAG298850.043.788428
GCAGAGT321450.040.7136739
AGAGTAC307100.033.32291410-11
AGTACGG346200.029.25855412-13
CAGAGTA316750.028.67520310-11
GAGTACG350150.025.68496912-13
TACGGGT71000.025.50699814-15
TACGGGC91950.024.86995514-15
GTACGGG355500.024.53425614-15
TACGGGG112750.024.21177114-15
TACGGGA132950.023.4408514-15
ACGGGAT30350.020.38564316-17
Produced by FastQC (version 0.11.5)