FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004923498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4014900
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA100600.250566639268724No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC96270.23978181274751548No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCG85190.21218461231911132No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC84570.21064036464170963No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC83780.20867269421405268No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCA81680.20344217788736954No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG80090.19948192981145232No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA77210.19230865027771551No Hit
GTATCAACGCAGAGTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTT76000.1892948765847219No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT75830.1888714538344666No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT75770.1887220105108471No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC69660.1735036987222596No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA68290.17009140949961393No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAG65940.1642382126578495No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTT61580.15337866447483126No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT61200.15243219009190764No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGA59900.14919425141348477No Hit
CTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAG57290.14269346683603576No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC55830.13905701262796086No Hit
CCTCAAGGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCA55440.13808563102443397No Hit
GGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGT53490.13322872300679967No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGT52440.13061346484345812No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT51480.12822237166554584No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC50720.12632942289969862No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTG50680.12622979401728562No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA50110.12481008244290019No Hit
CCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAGGG49450.12316620588308551No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG48010.11957956611621709No Hit
CTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCC47520.1183591123066577No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA47160.1174624523649406No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA46990.1170390296146853No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCG46000.11457321477496327No Hit
GTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACC45470.11325313208299087No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACG45390.11305387431816483No Hit
GTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTA45350.11295424543575183No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC44960.11198286383222497No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC44930.11190814217041521No Hit
GTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACAAACCGCCTG44880.11178360606739894No Hit
ACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAGGGCACAACCTCCAAG44080.10979102841913871No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC43960.10949214177189967No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG43290.10782335799148174No Hit
GAGTACGGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG43100.10735012080001992No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGC42920.10690179082916137No Hit
TATCAACGCAGAGTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTG42490.10583078034322149No Hit
GTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCC42080.10480958429848813No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT41940.1044608832100426No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAAT41920.10441106876883609No Hit
CTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCG41580.10356422326832548No Hit
GGTATCAACGCAGAGTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCT41150.10249321278238561No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT40800.10162146006127176No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC40500.10087424344317418No Hit
GAGTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACT40490.10084933622257092No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)