FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7129194
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA164800.047.941241
TCAACGC191500.040.386844
CAACGCA194700.039.9677965
ATCAACG196500.039.2986343
AACGCAG205400.037.764836
GGTATCA96200.037.0441931
TATCAAC236450.033.313022
ACGCAGA238300.032.2219357
CGCAGAG243650.031.4354428
GCAGAGT263200.029.0550869
GTACATG369850.027.1367511
TACATGG368200.026.0140152
ACATGGG363000.025.8458233
ATACGAC6150.025.1514823
AGAGTAC251500.023.99784510-11
CATGGGT73150.022.7724114
CATGGGA200350.021.4394264
CAGAGTA266050.020.97564110-11
AGTACAT290900.020.71440312-13
GAGTACA269900.020.47570612-13
Produced by FastQC (version 0.11.5)