FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004923525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2481847
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156550.6307802213432173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91660.3693217188650227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71980.2900259363288712No Hit
GTGCAAGACGATGACTTGCAAAATGTCGCAGCTGGAACGCAACATAGAGA40720.16407135492236224No Hit
GTGTGGCTCCTCGGCTTTGACAGAGTGCAAGACGATGACTTGCAAAATGT34260.13804235313458083No Hit
CCCTGAACCAGGGGGAATTCAAAGAGCTGGTGCGAAAAGATCTGCAAAAT32510.13099115295987224No Hit
CTCTAAGGGTGAGCTGAAGCAGCTGCTTACAAAGGAGCTTGCAAACACCA29470.11874221094209272No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28620.11531734228580569Clontech Universal Primer Mix Long (96% over 26bp)
CTTTATTATTCTGCAGGTACATGTCCAGGGGCCCAGCCTCTGGGCCCAGT27630.11132837761554197No Hit
GTCTTAGGGGGTGCCCTCCCCGAGGCCTGGCTTATGGTGGTGGCCAGGGC26500.10677531693130157No Hit
GTCCAGGGGCCCAGCCTCTGGGCCCAGTAACTCAGCTACTCTTTGTGGCT26060.1050024437445177No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)