FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4863400
Sequences flagged as poor quality0
Sequence length150
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC260650.535941933626681No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT136300.2802566106016367No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT129290.2658428260064975No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA101510.20872229304601717No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC94370.19404120574083975No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT90490.18606324793354442No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC87230.1793601184356623No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG84710.17417855821030553No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC84190.17310934737015257No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT83960.17263642719085415No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC82990.1706419377390303No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA80840.16622116214993626No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG73740.15162232183246288No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG72650.14938109141752684No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA71180.14635851461940208No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66880.13751696344121397No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC61390.12622856437882962No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG59760.12287699962988856No Hit
CTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTC58690.12067689270880454No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT55770.11467286260640705No Hit
ATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATC55730.11459061561870296No Hit
GTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGGTTAGCGCAAG55610.11434387465559075No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT53870.11076613069046345No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG53270.10953242587490232No Hit
GTATCAACGCAGAGTACGGGTCAACGTTAGAACATCAAACATTAAAGGGT52690.10833984455319325No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG51810.10653041082370358No Hit
GTATAGACCCGAAACCCGGTGATCTAGCCATGGGCAGGTTGAAGGTTGGG51070.10500884155117819No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA227300.091.453591
GGTATCA111800.071.468111
TATCAAC354850.058.9750022
ATCAACG370250.056.3470953
CGCAGAG374600.055.692778
AACGCAG378150.055.588616
ACGCAGA377800.055.3162887
GCAGAGT382850.054.2786259
CAACGCA388850.053.910925
TCAACGC395650.052.820664
CTATTGA38550.039.757189
GCTATTG40250.038.2618758
ACTGTGC136300.036.959038
TTCTCTG19550.036.810497
CTGTGCA128600.036.7607849
TTTAGGG43500.035.899542
CACTGTG137100.035.2211467
TCTCTGC19800.034.8918848
GGGCTAT46200.034.7361156
GGCTATT48200.032.9961747
Produced by FastQC (version 0.11.5)