FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004923540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3711968
Sequences flagged as poor quality0
Sequence length150
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC164250.44248765075561No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC112060.30188837834808924No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA102630.27648406451779756No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT96850.26091280959318613No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC85200.22952784075724791No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA84290.22707631100268105No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG80530.2169469133354598No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT70350.18952210794920646No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA70040.18868697143940896No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC67550.18197893947361615No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA66880.18017396701695704No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT66270.17853063388477486No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG65560.17661790187846446No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62070.1672158811713894No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT59050.15908003517271702No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC58740.1582448986629195No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT53520.14418227743342615No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC53420.1439128785592979No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG50930.13720484659350513No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC49630.1337026612298382No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC49300.13281364494521503No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC48190.12982331744239173No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT45270.12195687031784756No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG45020.121283373132527No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA43910.1182930456297037No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA43760.11788894731851138No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA42450.1143598220674316No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT41070.11064211760446212No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG40190.10827140751213374No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC39070.1052541401218976No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG38030.10245239183096407No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)