FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004923565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1158472
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55340.47769820936544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37040.31973150840072095No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG28580.24670427943014592No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26630.22987176211423324No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCT23330.20138596357961178No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT17820.1538233120869559No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTT17200.1484714347865119No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATATAG17030.1470039845589708No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA16570.14303323688444777No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA15500.13379693251110084No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA14870.12835873460903674No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT14610.12611439896691504No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT13970.12058988046323088No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC13560.11705073579680822No Hit
ATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACC13360.1153243237644069No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA13180.11377055293524574No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC13050.1126483851141849No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTT13030.11247574391094477No Hit
CCCTATAGAAGAGCTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC12870.11109461428502374No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATAT12750.11005876706558293No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT12590.1086776374396619No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT12510.10798707262670139No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT12390.1069512254072606No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC11850.10228991291977708No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG11700.10099510389547611No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)