FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004923607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200317
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49062.4491181477358386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13240.6609523904611192No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10490.5236699830768232No Hit
GCACCCTTTTTCCTGATATACTGAGGACACTCGGTCTCTAGCAATTTCTT9610.4797396127138485No Hit
CCCTCGCCATCCTTGCTGCCATTCTCCTGGTGGCCCTGCAGGCCCAGGCT7630.38089627939715553No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7230.36092792923216704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6410.3199928113939406No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT5930.2960307911959544No Hit
CTTCTGGGATGTCCGCTGCAATCTGCTCCGGGGCTGCAGCAACCTCATCA4670.23313048817624069No Hit
TCCCAGAAGTGGTTGTTTCCCTTGCATGGGACGAAAGCTTGGCTCCAAAG4440.22164868683137226No Hit
ACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCT4000.19968350164988494No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3960.19768666663338608No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT3780.18870090905914128No Hit
GGTCATCCCTGTAGACGGCATGGAAATTGCTAGAGACCGAGTGTCCTCAG3470.17322543768127516No Hit
CTCCTGCAATGCACGCTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTT2920.145768956204416No Hit
GGCTCAGCCTGGGCCTGCAGGGCCACCAGGAGAATGGCAGCAAGGATGGC2860.14277370367966774No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA2840.1417752861714183No Hit
GGCCCAGGCTGAGCCACTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCC2660.13278952859717347No Hit
GATTTCAATTTTCTTCACCATTTTCCGGAGGGAGGCCCCATAGCGGGTCC2550.12729823230180165No Hit
TGGCAAGGGTCCTCATGGCTGGGGTCACAGATCCTCTAGCTAGGCAGGGT2520.1258006060394275No Hit
GTCTTCATGCAGGAACCACAGTGCCAGATCCCCACAGCTCGTCTCTTCAT2370.11831247472755682No Hit
TCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTG2100.10483383836618959No Hit
CCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCT2080.10383542085794016No Hit
GATTACTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGG2030.1013393770873166No Hit
TCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAA2020.1008401683331919No Hit
CTTTCGTCCCATGCAAGGGAAACAACCACTTCTGGGATGTCCGCTGCAAT2020.1008401683331919No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)