FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004923608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200317
Sequences flagged as poor quality0
Sequence length150
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20221.0094001008401683No Hit
TCTTTATCACCAGAATGAGGAACTCCTGGAAGTTAACTGCACCATCAGTG16260.8117134342067822No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6710.334969074017682No Hit
CCCTCGCCATCCTTGCTGCCATTCTCCTGGTGGCCCTGCAGGCCCAGGCT6060.30252050499957567No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5960.2975284174583286No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4990.24910516830823146No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT4870.24311466325873493No Hit
CTTCTGGGATGTCCGCTGCAATCTGCTCCGGGGCTGCAGCAACCTCATCA4550.2271399831267441No Hit
TCCCAGAAGTGGTTGTTTCCCTTGCATGGGACGAAAGCTTGGCTCCAAAG3760.18770249155089183No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3530.17622069020602346No Hit
ACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCT3290.16423968010703036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3050.15225867000803725No Hit
GGCTCAGCCTGGGCCTGCAGGGCCACCAGGAGAATGGCAGCAAGGATGGC2450.12230614476055451No Hit
TGGCAAGGGTCCTCATGGCTGGGGTCACAGATCCTCTAGCTAGGCAGGGT2380.11881168348168154No Hit
CTCCTGCAATGCACGCTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTT2170.10832829964506256No Hit
GGCCCAGGCTGAGCCACTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCC2170.10832829964506256No Hit
CTTTCGTCCCATGCAAGGGAAACAACCACTTCTGGGATGTCCGCTGCAAT2060.10283700334969073No Hit
GGAACACAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCTAGAG2050.10233779459556604No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)