FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8152383
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC174460.21399877802600786No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA173190.21244095131448068No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG161390.19796665588454318No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158590.19453207730794786No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA135970.16678558894988127No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA131010.16070147832848383No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA127450.15633465699538407No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC124710.15297367653114433No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAAAAAAAAAAAAAAAAAAAAAAA118950.14590825774500535No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT110930.13607064339347158No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC106450.13057531767091904No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC104550.12824471077965793No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC102190.1253498516936704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92490.11345149019617944No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC89930.11031130406900656No Hit
TGAAATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTAC87190.10695032360476685No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC86490.10609167896061802No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC83510.10243630604695583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82450.10113607272867332No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA280150.064.9161761
GGTATCA162250.059.416871
ATCAACG363400.049.4372373
TCAACGC364100.049.3026244
TACAGAG79100.048.0562256
CAACGCA376050.047.6373525
AACGCAG383050.046.7613566
CGCAGAG389250.046.0162628
ACGCAGA391050.045.8044477
TATCAAC396700.045.5963252
GCAGAGT415750.043.1524359
AATACAG110950.035.3194734
ATACAGA112800.034.7880635
ACAGAGA121400.031.3115637
GAAATAC161850.031.2442252
TCTATAC67400.029.7117063
TAGGGGT41200.029.1985934
GTACATG53000.028.7201211
CAGAGAA131650.028.5455998
AAAGGTC45700.027.568397
Produced by FastQC (version 0.11.5)