FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8152383
Sequences flagged as poor quality0
Sequence length150
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC161540.19815065116543223No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA141140.17312729296452337No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG135270.16592694430573243No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA121300.1487908504789336No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA121180.14864365425422235No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC119330.14637437912325757No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119020.14599412220942024No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA116710.1431605948837291No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT115060.1411366467939497No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC93830.1150951813721215No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC91020.1116483364434669No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC86240.1057850201591363No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC86000.10549062770971385No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC83280.10215417994959264No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA282400.065.983221
GGTATCA157350.060.8011971
ATCAACG367650.050.173623
TCAACGC368650.049.9963264
CAACGCA382100.048.5754745
AACGCAG384600.048.4468466
CGCAGAG388450.047.8202258
ACGCAGA390650.047.697727
TATCAAC397950.046.4460952
GCAGAGT413950.044.8385479
ACTGTGC68650.030.0884698
TCTATAC59400.028.1101343
CAGAGTA424950.027.6903219
GAGTACG437650.026.6663281
TTAGGGC89650.026.572913
AGTACGG436700.026.4200152
CTGTGCA75500.026.4046789
TACGGGT115650.025.4519144
GTACATG51850.025.1801221
TAGGGCT104650.024.5511424
Produced by FastQC (version 0.11.5)