FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004923656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5298574
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA186600.070.9841161
GGTATCA76500.059.5819851
ATCAACG260600.050.3232153
TCAACGC261400.050.031684
CAACGCA263250.049.830285
AACGCAG269150.048.7402346
ACGCAGA271900.048.246367
TATCAAC276600.047.6058582
CGCAGAG282550.046.4050338
GCAGAGT299450.043.55159
AGAGTAC284200.035.09232710-11
AGTACGG321250.030.87747212-13
CAGAGTA293250.029.85196710-11
GAGTACG326000.026.7974212-13
TACGGGT66050.026.65429914-15
TACGGGA121250.025.53214614-15
GTACGGG341850.024.8712114-15
TACGGGC90600.024.68357314-15
TACGGGG111300.023.75335714-15
ACGGGAT28750.020.17424416-17
Produced by FastQC (version 0.11.5)